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expression plasmid ppicza  (Thermo Fisher)


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    Structured Review

    Thermo Fisher expression plasmid ppicza
    Expression Plasmid Ppicza, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ppicza+expression+plasmid/pmc08877874-34-9-15
    Average 90 stars, based on 1 article reviews
    expression plasmid ppicza - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Copsin, a Novel Peptide-based Fungal Antibiotic Interfering with the Peptidoglycan Synthesis
    Article Snippet: The coding sequences of the copsin precursor protein were amplified from cDNA by PCR with the Phusion high fidelity DNA polymerase (Thermo Scientific) according to standard protocols ( 49 ): forward primer, 5′-CCG GAATTC ATGAAACTTTCTACTTCTTTGCTCG-3′, and reverse primer, 5′-ACGC GTCGAC TTAACAACGAGGGCAGGGG-3′. .. The PCR product was cloned into the pPICZA expression plasmid (Invitrogen) containing a Zeocin resistance gene using the EcoRI and SalI (Thermo Scientific) restriction sites. ..

    Article Title: Copsin, a Novel Peptide-based Fungal Antibiotic Interfering with the Peptidoglycan Synthesis
    Article Snippet: The coding sequence of the copsin precursor protein was amplified from cDNA by PCR with the Phusion high-fidelity DNA polymerase (Thermo Scientific) according to standard protocols (Sambrook J, Russell D, 2001, Molecular cloning, 3rd edition). .. FP:5’CCGGAATTCATGAAACTTTCTACTTCTTTGCT CG-3’; RP:5’ACGCGTCGACTTAACAACGAGGGCAGGGG3’; The PCR product was cloned into the pPICZA expression plasmid (Life Technologies, Carlsbad, CA) containing a zeocin resistance gene using the EcoRI and SalI (Thermo Scientific) restriction sites. ..

    Clone Assay:

    Article Title: Copsin, a Novel Peptide-based Fungal Antibiotic Interfering with the Peptidoglycan Synthesis
    Article Snippet: The coding sequences of the copsin precursor protein were amplified from cDNA by PCR with the Phusion high fidelity DNA polymerase (Thermo Scientific) according to standard protocols ( 49 ): forward primer, 5′-CCG GAATTC ATGAAACTTTCTACTTCTTTGCTCG-3′, and reverse primer, 5′-ACGC GTCGAC TTAACAACGAGGGCAGGGG-3′. .. The PCR product was cloned into the pPICZA expression plasmid (Invitrogen) containing a Zeocin resistance gene using the EcoRI and SalI (Thermo Scientific) restriction sites. ..

    Article Title: Induction of antibacterial proteins and peptides in the coprophilous mushroom Coprinopsis cinerea in response to bacteria
    Article Snippet: .. The codon-optimized prepro-Copsin (CPP1) insert and the native prepro-CPP2 insert were cloned into the pPICZA expression plasmid (Invitrogen) using the restriction enzymes EcoRI / SalI (Thermo Scientific) [ ]. .. Both constructs were linearized with SacI and transformed into P. pastoris by electroporation as described previously [ , ].The standard methanol-limited fed-batch procedure was performed in a 3.6 l Labfors 5 bioreactor (Infors) according to the Pichia fermentation process guidelines of Invitrogen (Thermo Scientific) and using the process parameters and feed-in strategy as described [ ].

    Article Title: Copsin, a Novel Peptide-based Fungal Antibiotic Interfering with the Peptidoglycan Synthesis
    Article Snippet: The coding sequence of the copsin precursor protein was amplified from cDNA by PCR with the Phusion high-fidelity DNA polymerase (Thermo Scientific) according to standard protocols (Sambrook J, Russell D, 2001, Molecular cloning, 3rd edition). .. FP:5’CCGGAATTCATGAAACTTTCTACTTCTTTGCT CG-3’; RP:5’ACGCGTCGACTTAACAACGAGGGCAGGGG3’; The PCR product was cloned into the pPICZA expression plasmid (Life Technologies, Carlsbad, CA) containing a zeocin resistance gene using the EcoRI and SalI (Thermo Scientific) restriction sites. ..

    Expressing:

    Article Title: Copsin, a Novel Peptide-based Fungal Antibiotic Interfering with the Peptidoglycan Synthesis
    Article Snippet: The coding sequences of the copsin precursor protein were amplified from cDNA by PCR with the Phusion high fidelity DNA polymerase (Thermo Scientific) according to standard protocols ( 49 ): forward primer, 5′-CCG GAATTC ATGAAACTTTCTACTTCTTTGCTCG-3′, and reverse primer, 5′-ACGC GTCGAC TTAACAACGAGGGCAGGGG-3′. .. The PCR product was cloned into the pPICZA expression plasmid (Invitrogen) containing a Zeocin resistance gene using the EcoRI and SalI (Thermo Scientific) restriction sites. ..

    Article Title: Induction of antibacterial proteins and peptides in the coprophilous mushroom Coprinopsis cinerea in response to bacteria
    Article Snippet: .. The codon-optimized prepro-Copsin (CPP1) insert and the native prepro-CPP2 insert were cloned into the pPICZA expression plasmid (Invitrogen) using the restriction enzymes EcoRI / SalI (Thermo Scientific) [ ]. .. Both constructs were linearized with SacI and transformed into P. pastoris by electroporation as described previously [ , ].The standard methanol-limited fed-batch procedure was performed in a 3.6 l Labfors 5 bioreactor (Infors) according to the Pichia fermentation process guidelines of Invitrogen (Thermo Scientific) and using the process parameters and feed-in strategy as described [ ].

    Article Title: Copsin, a Novel Peptide-based Fungal Antibiotic Interfering with the Peptidoglycan Synthesis
    Article Snippet: The coding sequence of the copsin precursor protein was amplified from cDNA by PCR with the Phusion high-fidelity DNA polymerase (Thermo Scientific) according to standard protocols (Sambrook J, Russell D, 2001, Molecular cloning, 3rd edition). .. FP:5’CCGGAATTCATGAAACTTTCTACTTCTTTGCT CG-3’; RP:5’ACGCGTCGACTTAACAACGAGGGCAGGGG3’; The PCR product was cloned into the pPICZA expression plasmid (Life Technologies, Carlsbad, CA) containing a zeocin resistance gene using the EcoRI and SalI (Thermo Scientific) restriction sites. ..

    Plasmid Preparation:

    Article Title: Copsin, a Novel Peptide-based Fungal Antibiotic Interfering with the Peptidoglycan Synthesis
    Article Snippet: The coding sequences of the copsin precursor protein were amplified from cDNA by PCR with the Phusion high fidelity DNA polymerase (Thermo Scientific) according to standard protocols ( 49 ): forward primer, 5′-CCG GAATTC ATGAAACTTTCTACTTCTTTGCTCG-3′, and reverse primer, 5′-ACGC GTCGAC TTAACAACGAGGGCAGGGG-3′. .. The PCR product was cloned into the pPICZA expression plasmid (Invitrogen) containing a Zeocin resistance gene using the EcoRI and SalI (Thermo Scientific) restriction sites. ..

    Article Title: Induction of antibacterial proteins and peptides in the coprophilous mushroom Coprinopsis cinerea in response to bacteria
    Article Snippet: .. The codon-optimized prepro-Copsin (CPP1) insert and the native prepro-CPP2 insert were cloned into the pPICZA expression plasmid (Invitrogen) using the restriction enzymes EcoRI / SalI (Thermo Scientific) [ ]. .. Both constructs were linearized with SacI and transformed into P. pastoris by electroporation as described previously [ , ].The standard methanol-limited fed-batch procedure was performed in a 3.6 l Labfors 5 bioreactor (Infors) according to the Pichia fermentation process guidelines of Invitrogen (Thermo Scientific) and using the process parameters and feed-in strategy as described [ ].

    Article Title: Copsin, a Novel Peptide-based Fungal Antibiotic Interfering with the Peptidoglycan Synthesis
    Article Snippet: The coding sequence of the copsin precursor protein was amplified from cDNA by PCR with the Phusion high-fidelity DNA polymerase (Thermo Scientific) according to standard protocols (Sambrook J, Russell D, 2001, Molecular cloning, 3rd edition). .. FP:5’CCGGAATTCATGAAACTTTCTACTTCTTTGCT CG-3’; RP:5’ACGCGTCGACTTAACAACGAGGGCAGGGG3’; The PCR product was cloned into the pPICZA expression plasmid (Life Technologies, Carlsbad, CA) containing a zeocin resistance gene using the EcoRI and SalI (Thermo Scientific) restriction sites. ..

    Cloning:

    Article Title: Induction of antibacterial proteins and peptides in the coprophilous mushroom Coprinopsis cinerea in response to bacteria.
    Article Snippet: .. Cloning, production and purification of Copsin and CPP2 The codon-optimized prepro-Copsin (CPP1) insert and the native prepro-CPP2 insert were cloned into the pPICZA expression plasmid (Invitrogen) using the restriction enzymes EcoRI/SalI (Thermo Scientific) [47]. ..

    Purification:

    Article Title: Induction of antibacterial proteins and peptides in the coprophilous mushroom Coprinopsis cinerea in response to bacteria.
    Article Snippet: .. Cloning, production and purification of Copsin and CPP2 The codon-optimized prepro-Copsin (CPP1) insert and the native prepro-CPP2 insert were cloned into the pPICZA expression plasmid (Invitrogen) using the restriction enzymes EcoRI/SalI (Thermo Scientific) [47]. ..



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